Pronounced biomaterial dependency in cartilage regeneration using nonexpanded compared with expanded chondrocytes. (September 2013)

• Record Type: Journal Article
• Authors: Tsuchida, Anika I; Bekkers, Joris EJ; Beekhuizen, Michiel; Vonk, Lucienne A; Dhert, Wouter JA; Saris, Daniël BF; Creemers, Laura B
• Is Part Of: Regenerative medicine. Volume 8:Number 5(2013)
• Page Start: 583
• Page End: 595
• Publication Date: 2013-09
• Subjects: cartilage tissue engineering -- chondrocyte -- cytokine -- growth factors -- hydrogel; Cellular therapy -- Periodicals; Stem cells -- Transplantation -- Periodicals; Degeneration (Pathology) -- Treatment -- Periodicals; Regeneration (Biology) -- Periodicals; 611.018
• DOI: 10.2217/rme.13.44
• Languages: English
• ISSNs: 1746-0751

Abstract

Aim: We aimed to investigate freshly isolated compared with culture-expanded chondrocytes with respect to early regenerative response, cytokine production and cartilage formation in response to four commonly used biomaterials.Materials & methods: Chondrocytes were both directly and after expansion to passage 2, incorporated into four biomaterials: Polyactive™, Beriplast ®, HyStem ® and a type II collagen gel. Early cartilage matrix gene expression, cytokine production and glycosaminoglycan (GAG) and DNA content in response to these biomaterials were evaluated.Results: HyStem induced more GAG production, compared with all other biomaterials (p ≤ 0.001). Nonexpanded cells did not always produce more GAGs than expanded chondrocytes, as this was biomaterial-dependent. Cytokine production and early gene expression were not predictive for final regeneration.Conclusion: For chondrocyte-based cartilage treatments, the biomaterial best supporting cartilage matrix production will depend on the chondrocyte differentiation state and cannot be predicted from early gene expression or cytokine profile.