Genes involved in hemorrhagic transformations that follow recombinant t-PA treatment in stroke patients. (April 2013)

• Record Type: Journal Article
• Authors: Fernandez-Cadenas, Israel; Rio-Espinola, Alberto Del; Domingues-Montanari, Sophie; Mendioroz, Maite; Fernandez-Morales, Jessica; Penalba, Anna; Rubiera, Marta; Hernandez-Guillamon, Mar; Rosell, Anna; Delgado, Pilar; Chacon, Pilar; Ribo, Marc; Alvarez-Sabin, Jose; Molina, Carlos A; García-Arumi, Elena; Montaner, Joan
• Is Part Of: Pharmacogenomics. Volume 14:Number 5(2013)
• Page Start: 495
• Page End: 504
• Publication Date: 2013-04
• Subjects: hemorrhagic transformation -- microarrays -- rt-PA -- stroke -- transcription; Pharmacogenomics -- Periodicals; 615.1
• DOI: 10.2217/pgs.13.19
• Languages: English
• ISSNs: 1462-2416

Abstract

Aim: Despite the benefits of recombinant t-PA (rt-PA) for stroke patients some of them suffer from adverse hemorrhagic transformations (HTs) following treatment. Our objective is to study the transcriptomics of HTs patients.Methods: We studied by microarrays 11 blood samples from patients with stroke that had received rt-PA of whom six of them had suffered a HT. For replication step RNA was collected from 14 new subjects (seven with HT, seven without) and then analyzed by real-time PCR. Four proteins were measured by ELISA in 72 new subjects to analyze their role as potential protein biomarkers.Results: The microarray analysis revealed that 14 genes were altered among the HT patients. The replication study confirmed these results for six genes. Two of them ( BCL2 and OLFM4 ) are associated with apoptosis, whereas the other four ( LTF, LCN2 [also known as NGAL ], CEACAM8 and CRISP3 ) are involved in the regulation of neutrophil processes.Conclusion: Our data revealed that genes related to apoptosis and neutrophil regulation pathways could be associated with HTs after rt-PA. Original submitted 7 September 2012; Revision submitted 23 January 2013