Automated disposable small-scale bioreactor for high-throughput process development: implementation of the 24 bioreactor array. (June 2015)

• Record Type: Journal Article
• Authors: Bareither, Rachel; Goldfeld, Marina; Kistler, Chris; Tait, Andrew; Bargh, Neil; Oakeshott, Robert; O'Neill, Kristin; Hoshan, Linda; Pollard, David
• Is Part Of: Pharmaceutical bioprocessing. Volume 3:Number 3(2015)
• Page Start: 185
• Page End: 197
• Publication Date: 2015-06
• Subjects: Drugs -- Testing -- Periodicals; Drug development -- Periodicals; Clinical trials -- Periodicals; Biopharmaceutics -- Periodicals; 615.19
• DOI: 10.4155/pbp.14.64
• Languages: English
• ISSNs: 2048-9153

Abstract

Aim: A disposable reactor system (250 ml scale) has now been developed into a fully automated 24 reactor array which includes process parameter monitoring and control, advanced feed strategies, automated event triggering, robotic arm liquid handling and sample storage.Results: Process and analytical comparability was shown for a range of Pichia, Escherichia coli and CHO cell-culture bioprocesses up to pilot and commercial scale. This established the system as a scale-down model for process development and characterization of large-scale industrial processes.Conclusion: This work demonstrated the ability of the automated system to accelerate process development by executing a single statistical design of experiments, with a wider range of parameters, up to 3–5-times faster than conventional approaches.