Assessment of tumor antigen-loaded solid lipid nanoparticles as an efficient delivery system for dendritic cell engineering. (July 2013)

• Record Type: Journal Article
• Authors: Bhargava, Arpit; Mishra, Dinesh; Khan, Saba; Varshney, Subodh K; Banerjee, Smita; Mishra, Pradyumna K
• Is Part Of: Nanomedicine. Volume 8:Number 7(2013)
• Page Start: 1067
• Page End: 1084
• Publication Date: 2013-07
• Subjects: cancer immunotherapy -- gastrointestinal cancer -- immune tolerance -- nanomedicine -- translational oncology; Nanotechnology -- Periodicals; Medical technology -- Periodicals; Nanotechnology -- Therapeutic use -- Periodicals; 610.28
• DOI: 10.2217/nnm.12.164
• Languages: English
• ISSNs: 1743-5889

Abstract

Aim: The work attempts to overcome tumor-associated immune tolerance using a surface-modified solid lipid nanoparticle (SLNP) delivery system for dendritic cell (DC) immunotherapy.Materials & methods: Different formulations of SLNPs (SLNPs-alone, cationic SLNPs and mannosylated SLNPs) were prepared using tumor cell lysates. Prepared nanoparticles were characterized and their ability to activate DCs to induce a tumor cell-specific response was assessed.Results: SLNPs induced a strong phagocytic signal to DCs without any significant toxicity. Comparatively, mannosylated SLNPs evoked an optimum and effective cell-mediated immune response with no significant toxicity.Conclusion: Surface-modified SLNPs may play a pivotal role in designing a clinically translatable DC-based immunotherapy for gastrointestinal malignancies. This novel approach may also facilitate the treatment of residual disease, following standard therapy. Original submitted 9 February 2012; Revised submitted 19 August 2012; Published online 14 January 2013