Polyelectrolyte multilayer coating with two regulatory molecules on titanium: construction and its biological effects. (May 2013)

• Record Type: Journal Article
• Authors: Luo, Qiaojie; Huang, Ying; Deng, Xuliang; Zhang, Jianxiang; Li, Xiaohui; Zhao, Shifang; Li, Xiaodong
• Is Part Of: Nanomedicine. Volume 8:Number 5(2013)
• Page Start: 739
• Page End: 755
• Publication Date: 2013-05
• Subjects: bFGF -- osseointegration -- polyelectrolyte multilayer -- RGD -- synergistic effect; Nanotechnology -- Periodicals; Medical technology -- Periodicals; Nanotechnology -- Therapeutic use -- Periodicals; 610.28
• DOI: 10.2217/nnm.12.151
• Languages: English
• ISSNs: 1743-5889

Abstract

Aim: This study aimed at constructing a novel disulfide-crosslinked collagen I/hyaluronic acid polyelectrolyte multilayer (PEM) coating incorporated with bFGF and arginine–glycine–aspartic acid on titanium via the layer-by-layer technique, and evaluating its biological effects.Materials & methods: The surface topography and components, thickness, degradation behaviors and bFGF release profiles of the PEM coating were investigated. The effects of the PEM coating on osteoprogenitor cell growth and bone implant interfacial binding strength in the rabbit femur model were evaluated separately.Results: The formation of disulfide bonds improved the stability of the PEM coating, resulting in a coating that can release bFGF in a slow and sustained manner. Biological evaluations revealed that the resultant PEM coating on titanium promoted various cell behaviors and enhanced the binding strength.Conclusion: The employed cotreatment regimen enabled bFGF and arginine–glycine–aspartic acid to have a synergistic effect on the cell responses, which, in turn, improved the osseointegration. Original submitted 26 January 2012; Revised submitted 10 June 2012; Published online 5 February 2013