Cytokine and chemokine receptor profile of peripheral blood mononuclear cells during treatment with infliximab in patients with active rheumatoid arthritis. Issue 6 (12th May 2004)

• Record Type: Journal Article
• Authors: Nissinen, R; Leirisalo-Repo, M; Peltomaa, R; Palosuo, T; Vaarala, O
• Is Part Of: Annals of the rheumatic diseases. Volume 63:Issue 6(2004)
• Page Start: 681
• Page End: 687
• Publication Date: 2004-05-12
• Subjects: infliximab -- rheumatoid arthritis -- chemokine receptors -- cytokines -- tumour necrosis factor α; ACR, American College of Rheumatology -- AFA, antifilaggrin antibodies -- BSA, bovine serum albumin -- CCP, cyclic citrullinated peptide -- CRP, C reactive protein -- DMARD, disease modifying antirheumatic drug -- ELISA, enzyme linked immunosorbent assay -- FACS, fluorescence activated cell sorter -- IFNγ, interferon γ -- IL, interleukin -- PBMC, peripheral blood mononuclear cells -- PBS, phosphate buffered saline -- PHA, phytohaemagglutinin -- RA, rheumatoid arthritis -- RF, rheumatoid factor -- RT-PCR, reverse transcriptase-polymerase chain reaction -- SAA, serum amyloid A -- T, Tween -- TNFα, tumour necrosis factor α; Rheumatism -- Periodicals; 616.723005
• DOI: 10.1136/ard.2003.008599
• Languages: English
• ISSNs: 0003-4967

Abstract

Abstract : Objectives: To analyse immunological changes during treatment with a monoclonal anti-tumour necrosis factor α (TNFα) antibody, infliximab, in patients with rheumatoid arthritis (RA). Methods: 25 patients with RA and 5 patients with other arthritides were studied during the first 6 weeks of treatment with infliximab. At the start of treatment and after 2 and 6 weeks, spontaneous expression of CCR3 and CCR5 on peripheral blood T cells and monocytes was studied by flow cytometry. The secretion and mRNA expression of interferon γ (IFNγ), interleukin (IL)4, IL5, and TNFα from phytohaemagglutinin (PHA) stimulated peripheral blood mononuclear cells was measured with an ELISA and RT-PCR. Plasma levels of C reactive protein, serum amyloid protein A, rheumatoid factor, and antibodies to filaggrin and citrullinated cyclic peptide were measured with an ELISA. Results: The number of CD4 T cells and CD14 monocytes expressing CCR3 (p = 0.013, p = 0.009, respectively) and CD8 T cells expressing CCR5 (p = 0.040) as well as PHA stimulated secretion of IL4 and IFNγ (p<0.05) increased during treatment in patients with RA. 15 (60%) patients with RA achieved clinical response (at least ACR20) during the first 2 weeks. The number of T cells expressing CCR3 and CCR5 was higher before treatment in non-responders than in responders (p<0.05). The number of T cells increased in responders. Conclusion: Increase in secretion of Th1 and Th2 cytokines together with induced expression of chemokine receptors on T cells and monocytes suggest restoration of peripheral cell mediated immunity and blockade of the accumulation of inflammatory cells in joints as response to treatment.