Simultaneous determination of centchroman and tamoxifen along with their metabolites in rat plasma using LC–MS/MS. (May 2015)

• Record Type: Journal Article
• Authors: Rama Raju, Kanumuri Siva; Taneja, Isha; Singh, Sheelendra Pratap; Tripathi, Amit; Mishra, Durga Prasad; Hussain, K Mahaboob; Gayen, Jiaur Rahman; Singh, Shio Kumar; Wahajuddin, Muhammad
• Is Part Of: Bioanalysis. Volume 7:Number 8(2015)
• Page Start: 967
• Page End: 979
• Publication Date: 2015-05
• Subjects: Drugs -- Analysis -- Periodicals; 615.19005
• DOI: 10.4155/bio.14.253
• Languages: English
• ISSNs: 1757-6180

Abstract

Aim: Tamoxifen and centchroman are two non-steroidal, selective estrogen receptors modulators, intended for long term therapy in the woman. Because of their wide spread use, there is a possibility of co-prescription of these agents.Materials & Methods : We studied the probable pharmacokinetic interaction between these agents in breast cancer model rats. A simple, sensitive and rapid LC-ESI-MS/MS method was developed and validated for the simultaneous determination of tamoxifen, centchroman and their active metabolites.Results: The method was linear over a range of 0.2–200 ng/ml. All validation parameters met the acceptance criteria according to regulatory guidelines.Conclusion: LC–MS/MS method for determination of tamoxifen, centchroman and their metabolites was developed and validated. Results show the potential of drug–drug interaction upon co-administration these two marketed drugs.