Antibody-free workflows for protein quantification by LC–MS/MS. (April 2015)

• Record Type: Journal Article
• Authors: Wilffert, Daniel; Bischoff, Rainer; van de Merbel, Nico C
• Is Part Of: Bioanalysis. Volume 7:Number 6(2015)
• Page Start: 763
• Page End: 779
• Publication Date: 2015-04
• Subjects: Drugs -- Analysis -- Periodicals; 615.19005
• DOI: 10.4155/bio.14.308
• Languages: English
• ISSNs: 1757-6180

Abstract

Antibody-free approaches for quantitative LC–MS/MS-based protein bioanalysis are reviewed and critically evaluated, and compared with the more widely used immunoaffinity-based approaches. Antibody-free workflows will be divided into four groups and discussed in the following order: direct analysis of signature peptides after proteolytic digestion; enrichment of target proteins and signature peptides by fractionated protein precipitation; enrichment of target proteins and signature peptides by reversed-phase and ion-exchange solid-phase extraction; and enrichment of target proteins and signature peptides by (antibody-free) affinity-solid-phase extraction.