Quantitation of 25-hydroxyvitamin D2 and D3 in serum and plasma by LCMS/MS. (January 2015)

• Record Type: Journal Article
• Authors: Lee, Dayong; Garrett, Timothy J; Goldberger, Bruce A; Bazydlo, Lindsay AL
• Is Part Of: Bioanalysis. Volume 7:Number 2(2015)
• Page Start: 167
• Page End: 178
• Publication Date: 2015-01
• Subjects: Drugs -- Analysis -- Periodicals; 615.19005
• DOI: 10.4155/bio.14.229
• Languages: English
• ISSNs: 1757-6180

Abstract

Objective: We developed and validated an analytical method for quantifying 25-hydroxyvitamin D2 (25OHD2 ) and 25-hydroxyvitamin D3 (25OHD3 ) in serum and plasma.Methods: Samples, pretreated with zinc sulfate and methanol, were extracted with hexane. Separation was achieved via UHPLC and 25OHD quantification was accomplished by a triple quadrupole mass spectrometer.Results: Imprecision was 3.6–15.1%CV and bias 88.0–126.0%. Extraction efficiency was 76.5–110.5%, whereas the matrix effect ranged from −46.7 to −32.0%. The method was applied to authentic specimens. The results showed no significant difference between serum and plasma; strong correlation with paired values from an external laboratory; and analyte stability for 15 days.Conclusion: This method provides reliable and accurate measurement of 25OHD for use in clinical practice.