A novel approach for quantitation of glucosylceramide in human dried blood spot using LC–MS/MS. (July 2015)

• Record Type: Journal Article
• Authors: Ji, Allena Ji; Wang, Haixing; Ziso-Qejvanaj, Enida; Zheng, Kefei; Chung, Lee Lee; Foley, Timothy; Chuang, Wei-Lien; Richards, Susan; Sung, Crystal
• Is Part Of: Bioanalysis. Volume 7:Number 12(2015)
• Page Start: 1483
• Page End: 1496
• Publication Date: 2015-07
• Subjects: Drugs -- Analysis -- Periodicals; 615.19005
• DOI: 10.4155/bio.15.77
• Languages: English
• ISSNs: 1757-6180

Abstract

Background: Glucosylceramide, an efficacy biomarker for Gaucher Type 1 disease, exhibits poor solubility in polar solvents and whole blood which makes it difficult to prepare a homogenous blood standard.Results: We developed a novel method using standard addition approach by spiking a small volume of analyte solution on the surface of prespotted dried blood spot. The whole spots were punched out for subsequent extraction and LC–MS/MS analysis. The assay performance met all validation acceptance criteria. Glucosylceramide concentrations in 50 paired plasma and dry blood spot samples obtained from Gaucher Type 1 patients were tested and the results demonstrated the feasibility of using the DBS method for clinical biomarker monitoring.Conclusion: The new approach greatly improves assay precision and accuracy.