Determination of thailandepsin B in rat plasma with a LC–MS/MS method. (January 2015)

• Record Type: Journal Article
• Authors: Cheng, Zhongzhe; Ding, Lian; Zhang, Yang; Chen, Guiying; Chen, Chang; Cheng, Yi-Qiang; Jiang, Hongliang
• Is Part Of: Bioanalysis. Volume 7:Number 1(2015)
• Page Start: 39
• Page End: 52
• Publication Date: 2015-01
• Subjects: Drugs -- Analysis -- Periodicals; 615.19005
• DOI: 10.4155/bio.14.264
• Languages: English
• ISSNs: 1757-6180

Abstract

Background: Thailandepsin B (TDP-B) is a potent histone deacetylase inhibitor under development. A reliable bioanalytical method for the quantification of TDP-B in plasma samples is required.Results: The stabilizer mixture containing hydrochloric acid, formic acid and dichlorvos was applied to stabilize TDP-B in matrix samples. The method validation was conducted over the curve range of 1.00 to 1000 ng/ml. The intrabatch and interbatch precision and accuracy of the quality control samples showed ≤10.9% relative standard deviation and -6.7% to 15.0% relative error. Moreover, a possible metabolite M of TDP-B was tentatively characterized.Conclusion: A reliable LC–MS/MS method was developed and validated for the quantification of TDP-B and was successfully applied to a rat pharmacokinetic study.