A practical approach to automate randomized design of experiments for ligand-binding assays. (March 2014)

• Record Type: Journal Article
• Authors: Tsoi, Jennifer; Patel, Vimal; Shih, Judy
• Is Part Of: Bioanalysis. Volume 6:Number 5(2014)
• Page Start: 705
• Page End: 713
• Publication Date: 2014-03
• Subjects: Drugs -- Analysis -- Periodicals; 615.19005
• DOI: 10.4155/bio.13.317
• Languages: English
• ISSNs: 1757-6180

Abstract

Background: Design of experiments (DOE) is utilized in optimizing ligand-binding assay by modeling factor effects. To reduce the analyst's workload and error inherent with DOE, we propose the integration of automated liquid handlers to perform the randomized designs.Results: A randomized design created from statistical software was imported into custom macro converting the design into a liquid-handler worklist to automate reagent delivery. An optimized assay was transferred to a contract research organization resulting in a successful validation.Conclusion: We developed a practical solution for assay optimization by integrating DOE and automation to increase assay robustness and enable successful method transfer. The flexibility of this process allows it to be applied to a variety of assay designs.