Novel sandwich immunoassays for the measurement of total and active FGF21. (December 2014)

• Record Type: Journal Article
• Authors: Umberger, Tara S; Sloan, John H; Chen, Jinbiao; Cheng, Christine; Siegel, Robert W; Qian, Yuewei; Troutt, Jason S; Konrad, Robert J
• Is Part Of: Bioanalysis. Volume 6:Number 24(2014)
• Page Start: 3283
• Page End: 3293
• Publication Date: 2014-12
• Subjects: Drugs -- Analysis -- Periodicals; 615.19005
• DOI: 10.4155/bio.14.241
• Languages: English
• ISSNs: 1757-6180

Abstract

Background: FGF21 is involved in glucose and lipid metabolism. Our objective was to develop two novel sandwich immunoassays that measure active and total levels of FGF21 in human plasma.Results: Both immunoassays utilized affinity-optimized monoclonal antibodies generated specifically for either the mid-domain or the intact C-terminus of the respective FGF21 peptides. Total FGF21 levels measured from normal human plasma samples ranged from 42 to 462 pg/ml, while active FGF21 levels ranged from 11 to 399 pg/ml. The data also suggested no significant differences in the concentrations of active FGF21 when measured from pre- and post-prandial samples.Conclusion : We have successfully developed sensitive assays to measure active and total FGF21, which show the majority of total FGF21 in plasma is active FGF21.