CYP1A2 phenotyping in dried blood spots and microvolumes of whole blood and plasma. (November 2014)

• Record Type: Journal Article
• Authors: De Kesel, Pieter MM; Lambert, Willy E; Stove, Christophe P
• Is Part Of: Bioanalysis. Volume 6:Number 22(2014)
• Page Start: 3011
• Page End: 3024
• Publication Date: 2014-11
• Subjects: Drugs -- Analysis -- Periodicals; 615.19005
• DOI: 10.4155/bio.14.149
• Languages: English
• ISSNs: 1757-6180

Abstract

Background: Phenotyping, using caffeine as probe substrate, is a proper method to assess CYP1A2 activity. We evaluated the utility of dried blood spots (DBS) for CYP1A2 phenotyping.Results: LC–MS/MS methods were developed and validated for quantitation of caffeine and its metabolite paraxanthine in DBS, whole blood and plasma. All parameters met the pre-established criteria. While recovery, matrix effects and precision were unaffected by hematocrit (Hct), there was a Hct effect on accuracy, although for the evaluated Hct interval (0.36–0.50) it remained within acceptable limits. The phenotyping methods were successfully applied in healthy volunteers.Conclusion: Excellent method performance and highly comparable phenotyping indices in DBS, whole blood and plasma, combined with the benefits of DBS sampling, illustrate the suitability of DBS-based CYP1A2 phenotyping.