Untargeted profiling of urinary steroid metabolites after testosterone ingestion: opening new perspectives for antidoping testing. (October 2014)

• Record Type: Journal Article
• Authors: Boccard, Julien; Badoud, Flavia; Jan, Nicolas; Nicoli, Raul; Schweizer, Carine; Pralong, François; Veuthey, Jean-Luc; Baume, Norbert; Rudaz, Serge; Saugy, Martial
• Is Part Of: Bioanalysis. Volume 6:Number 19(2014)
• Page Start: 2523
• Page End: 2536
• Publication Date: 2014-10
• Subjects: doping analysis -- multiblock data analysis -- steroidomics -- testosterone -- UHPLC-QTOF/MS -- urine; Drugs -- Analysis -- Periodicals; 615.19005
• DOI: 10.4155/bio.14.200
• Languages: English
• ISSNs: 1757-6180

Abstract

Aim: Antidoping procedures are expected to greatly benefit from untargeted metabolomic approaches through the discovery of new biomarkers of prohibited substances abuse.Results: Endogenous steroid metabolites were monitored in urine samples from a controlled elimination study of testosterone undecanoate after ingestion. A platform coupling ultra-high pressure LC with high-resolution quadrupole TOF MS was used and high between-subject metabolic variability was successfully handled using a multiblock data analysis strategy. Links between specific subsets of metabolites and influential genetic polymorphisms of the UGT2B17 enzyme were highlighted.Conclusion: This exploratory metabolomic strategy constitutes a first step toward a better understanding of the underlying patterns driving the high interindividual variability of steroid metabolism. Promising biomarkers were selected for further targeted study.