Multidimensional gas chromatography methods for bioanalytical research. (September 2014)

• Record Type: Journal Article
• Authors: Wong, Yong Foo; Hartmann, Constanze; J Marriott, Philip
• Is Part Of: Bioanalysis. Volume 6:Number 18(2014)
• Page Start: 2461
• Page End: 2479
• Publication Date: 2014-09
• Subjects: Drugs -- Analysis -- Periodicals; 615.19005
• DOI: 10.4155/bio.14.186
• Languages: English
• ISSNs: 1757-6180

Abstract

Multidimensional gas chromatography (MDGC) methods are high-resolution volatile chemical separation techniques, and comprise classical heart-cutting MDGC and its more recent incarnation, comprehensive 2D GC. Although available for a long period, MDGC approaches are still not widely practiced in the field of bioanalysis, possibly reflecting the general preference for regular GC versus MDGC approaches. With the recent introduction of '-omic' techniques that emphasize global nontargeted profiling of metabolites within living systems, it is evident that MDGC is gaining momentum as a separation tool, since it offers very high resolution. By untangling metabolites within highly complex biological matrices, and expanding the metabolic coverage, MDGC plays a frontline role in '-omics' based studies. This review highlights state-of-the-art MDGC approaches, and summarizes the recent developments in bioanalytics.