Assessment of cardiovascular disease risk using immunosensors for determination of C-reactive protein levels in serum and saliva: a pilot study. (June 2014)

• Record Type: Journal Article
• Authors: Justino, Celine IL; Duarte, Kátia; Lucas, Susana; Chaves, Paulo; Bettencourt, Paulo; Freitas, Ana Cristina; Pereira, Ruth; Cardoso, Susana; Duarte, Armando C; Rocha-Santos, Teresa AP
• Is Part Of: Bioanalysis. Volume 6:Number 11(2014)
• Page Start: 1459
• Page End: 1470
• Publication Date: 2014-06
• Subjects: Drugs -- Analysis -- Periodicals; 615.19005
• DOI: 10.4155/bio.14.12
• Languages: English
• ISSNs: 1757-6180

Abstract

Background: Disposable immunosensors based on field effect transistors with single-walled carbon nanotubes (NTFET) were applied for the first time to clinical samples of undiluted blood serum and saliva for the determination of C-reactive protein (CRP), and validated by comparison with ELISA.Results: The NTFET showed comparable analytical performance with the ELISA when applied to clinical samples, which means that NTFET can be used as an alternative to ELISA. Also, a high correlation between the serum and salivary CRP levels was found with the NTFET, which means that saliva could be used based on a noninvasive sampling as an alternative fluid to blood serum. The establishment of a new range of CRP levels based on saliva was also found.Conclusion: The monitoring of CRP in saliva samples by disposable immunosensors could be a valuable approach for the improvement of healthcare services, considering the worldwide increased incidence of cardiovascular diseases.