Transcriptome Characterization and Identification of Molecular Markers (SNP, SSR, and Indels) in the Medicinal Plant Sarcandra glabra spp. (8th July 2021)

• Record Type: Journal Article
• Authors: Xu, Yanqin; Tian, Shuyun; Li, Renqing; Huang, Xiaofang; Li, Fengqin; Ge, Fei; Huang, Wenzhen; Zhou, Yin
• Other Names: Hu Biaolin Academic Editor.
• Is Part Of: BioMed research international. Volume 2021(2021)
• Publication Date: 2021-07-08
• Subjects: Medicine -- Periodicals; Biology -- Periodicals; Biotechnology -- Periodicals; Life sciences -- Periodicals; 610.5
• DOI: 10.1155/2021/9990910
• Languages: English
• ISSNs: 2314-6133

Abstract

Abstract : Sarcandra glabra has significant metabolically active bioingredients of pharmaceutical importance. The deficiency of molecular markers for S. glabra is a hindrance in molecular breeding for genetic improvement. In this study, 57.756 million pair-end reads were generated by transcriptome sequencing in S. glabra (Thunb.) Nakai and its subspecies S. glabra ssp. brachystachys . A total of 141, 954 unigenes with 646.63 bp average length were assembled. A total of 25, 620 simple sequence repeats, 726, 476 single nucleotide polymorphisms, and 42, 939 insertions and deletions were identified, and the associated unigenes and differentially expressed genes were characterized. This work enhanced the molecular marker resources and will facilitate molecular breeding and gene mining in S. glabra spp.