Visfatin facilitates gastric cancer malignancy by targeting snai1 via the NF-κB signaling. (October 2021)

• Record Type: Journal Article
• Authors: Cao, D; Chu, L; Xu, Z; Gong, J; Deng, R; Wang, B; Zhou, S
• Is Part Of: Human & experimental toxicology. Volume 40:Number 10(2021)
• Page Start: 1646
• Page End: 1655
• Publication Date: 2021-10
• Subjects: Visfatin -- NF-κB -- snai1 -- GC -- EMT; Toxicology -- Periodicals; 615.9
• DOI: 10.1177/09603271211006168
• Languages: English
• ISSNs: 0960-3271

Abstract

Background: Visfatin acts as an oncogenic factor in numerous tumors through a variety of cellular processes. Visfatin has been revealed to promote cell migration and invasion in gastric cancer (GC). Snai1 is a well-known regulator of EMT process in cancers. However, the relationship between visfatin and snai1 in GC remains unclear. The current study aimed to explore the role of visfatin in GC. Methods: The RT-qPCR and western blot analysis were used to measure RNA and protein levels, respectively. The cell migration and invasion were tested by Trans-well assays and western blot analysis. Results: Visfatin showed upregulation in GC cells. Additionally, Visfatin with increasing concentration facilitated epithelial-mesenchymal transition (EMT) process by increasing E-cadherin and reducing N-cadherin and Vimentin protein levels in GC cells. Moreover, endogenous overexpression and knockdown of visfatin promoted and inhibited migratory and invasive abilities of GC cells, respectively. Then, we found that snai1 protein level was positively regulated by visfatin in GC cells. In addition, visfatin activated the NF-κB signaling to modulate snai1 protein expression. Furthermore, the silencing of snai1 counteracted the promotive impact of visfatin on cell migration, invasion and EMT process in GC. Conclusion: Visfatin facilitates cell migration, invasion and EMT process by targeting snai1 via the NF-κB signaling, which provides a potential insight for the treatment of GC.