Mediation effect of AhR expression between polycyclic aromatic hydrocarbons exposure and oxidative DNA damage among Chinese occupational workers. (December 2018)

• Record Type: Journal Article
• Authors: Liu, Yanli; Zhang, Hongjie; Zhang, Huitao; Niu, Yingying; Fu, Ye; Nie, Jisheng; Yang, Aimin; Zhao, Jinzhu; Yang, Jin
• Is Part Of: Environmental pollution. Volume 243(2018)Part B
• Page Start: 972
• Page End: 977
• Publication Date: 2018-12
• Subjects: Polycyclic aromatic hydrocarbons -- 1-hydroxypyrene -- Oxidative DNA damage -- 8-hydroxydeoxyguanosine -- Aryl hydrocarbon receptor; Pollution -- Periodicals; Pollution -- Environmental aspects -- Periodicals; Environmental Pollution -- Periodicals; Pollution -- Périodiques; Pollution -- Aspect de l'environnement -- Périodiques; Pollution -- Effets physiologiques -- Périodiques; Pollution; Pollution -- Environmental aspects; Periodicals; Electronic journals; 363.73
• DOI: 10.1016/j.envpol.2018.09.014
• Languages: English
• ISSNs: 0269-7491

Abstract

Abstract: Polycyclic aromatic hydrocarbons (PAH) are well-known to be carcinogenic and the mechanisms that it contributes to oxidative DNA damage and aryl hydrocarbon receptor (AhR)-dependent induction are also well understood. However, little is known about the associations between PAH exposure, AhR expression, and oxidative DNA damage. We investigated their associations of AhR expression and oxidative DNA damage related to PAH exposure among 310 workers from a coke-oven plant in China. Urine biomarkers of PAH exposure (2-hydroxynaphthalene, 2-NAP; 2-hydroxyfluorene, 2-FLU; 9-hydroxyphenanthren, 9-PHE; and 1-hydroxypyrene, 1-OHP) and a marker of oxidative damage (8-hydroxy- 2′- deoxyguanosine, 8-OHdG) were measured by high performance liquid chromatography. AhR expression in venous blood was measured by reverse transcription -polymerase chain reaction. The results showed that increasing levels of urinary 1-OHP was positively associated with high 8-OHdG (OR (95% CI) was 4.01 (1.41–11.45) for 4th quartile, compared with 1st quartile, P for trend = 0.013). The similar associations were also found between urinary 1-OHP and high- AhR expressions (4th vs. 1st quartile = 3.50, 95% CI: 1.24–9.87, P for trend = 0.029). A significant association between AhR expression and high 8-OHdG was also found (4th vs. 1st quartile = 2.44, 95% CI: 1.05–5.70, P for trend = 0.027). In addition, mediation analysis showed the AhR expression could explain 35.9% of the association of oxidative DNA damage related to PAH exposure. Our findings implicated that the association between PAH exposure and oxidative DNA damage may be mediated by AhR expression among Chinese occupational workers. Graphical abstract: Image 1 Highlights: The linear dose-response associations between urinary 1-OHP and AhR expressions were observed among participants. Increasing levels of urinary 1-OHP prospectively associated with high 8-OHdG were also observed. A positive linear relationship between urinary 8-OHdG and AhR expressions was also found. AhR expression mediated the effect of oxidative DNA damage related to PAHs exposure.