Generation of Human-Induced Pluripotent Stem Cells by a Nonintegrating RNA Sendai Virus Vector in Feeder-Free or Xeno-Free Conditions. (22nd March 2012)

• Record Type: Journal Article
• Authors: MacArthur, Chad C.; Fontes, Andrew; Ravinder, Namritha; Kuninger, David; Kaur, Jasmeet; Bailey, Matthew; Taliana, Antje; Vemuri, Mohan C.; Lieu, Pauline T.
• Other Names: Verma Paul Academic Editor.
• Is Part Of: Stem cells international. Volume 2012(2012)
• Publication Date: 2012-03-22
• Subjects: Stem Cells -- Periodicals; Stem Cells -- Therapeutic use -- Periodicals; Stem Cells -- Transplantation -- Periodicals; 616.0277405
• DOI: 10.1155/2012/564612
• Languages: English
• ISSNs: 1687-966X

Abstract

Abstract : The generation of induced pluripotent stem cells (iPSCs) from somatic cells has enabled the possibility of providing unprecedented access to patient-specific iPSC cells for drug screening, disease modeling, and cell therapy applications. However, a major obstacle to the use of iPSC for therapeutic applications is the potential of genomic modifications caused by insertion of viral transgenes in the cellular genome. A second concern is that reprogramming often requires the use of animal feeder layers and reagents that contain animal origin products, which hinder the generation of clinical-grade iPSCs. Here, we report the generation of iPSCs by an RNA Sendai virus vector that does not integrate into the cells genome, providing transgene-free iPSC line. In addition, reprogramming can be performed in feeder-free condition with StemPro hESC SFM medium and in xeno-free (XF) conditions. Generation of an integrant-free iPSCs generated in xeno-free media should facilitate the safe downstream applications of iPSC-based cell therapies.