Modeling, Simulation, and Kinetic Studies of Solvent-Free Biosynthesis of Benzyl Acetate. (3rd October 2013)

• Record Type: Journal Article
• Authors: Garlapati, Vijay Kumar; Kumari, Annapurna; Mahapatra, Paramita; Banerjee, Rintu
• Other Names: Cruz Jose M. Academic Editor.
• Is Part Of: Journal of chemistry. Volume 2013(2013)
• Publication Date: 2013-10-03
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1155/2013/451652
• Languages: English
• ISSNs: 2090-9063

Abstract

Abstract : Solvent-free biosynthesis of benzyl acetate through immobilized lipase-mediated transesterification has been modeled and optimized through statistical integrated artificial intelligence approach. A nonlinear response surface model has been successfully developed based on central composite design with transesterification variables, namely, molarity of alcohol, reaction time, temperature, and immobilized lipase amount as input variables and molar conversion (%) as an output variable. Statistical integrated genetic algorithm optimization approach results in an optimized molar conversion of 96.32% with the predicted transesterification variables of 0.47 M alcohol molarity in a reaction time of 13.1 h, at 37.5°C using 13.31 U of immobilized lipase. Immobilized lipase withstands more than 98% relative activity up to 6 recycles and maintains 50% relative activity until 12 recycles. The kinetic constants of benzyl acetate, namely, K m and V m a x were found to be 310 mM and 0.10 mmol h −1 g −1, respectively.