Isolation, Identification, and Characterization of a Cellulolytic Bacillus amyloliquefaciens Strain SS35 from Rhinoceros Dung. (23rd May 2013)

• Record Type: Journal Article
• Authors: Singh, Shuchi; Moholkar, Vijayanand S.; Goyal, Arun
• Other Names: Brusetti L. Academic Editor.; Kreth J. Academic Editor.; Lai M. C. Academic Editor.; Maupin-Furlow J. Academic Editor.
• Is Part Of: ISRN microbiology. Volume 2013(2013)
• Publication Date: 2013-05-23
• Subjects: Microbiology -- Periodicals; Microbiology; Microbiological Phenomena; Fulltext; Internet Resources; Periodicals; 579
• DOI: 10.1155/2013/728134
• Languages: English
• ISSNs: 2090-7478

Abstract

Abstract : Cellulose hydrolyzing bacteria were isolated from rhinoceros dung and tested for clear zone formation around the colonies on the agar plates containing the medium amended with carboxymethylcellulose as a sole carbon source. Isolates were further screened on the basis of carboxymethylcellulase production in liquid medium. Out of 36 isolates, isolate no. 35 exhibited maximum enzyme activity of 0.079 U/mL and was selected for further identification by using conventional biochemical tests and phylogenetic analyses. This was a Gram-positive, spore forming bacterium with rod-shaped cells. The isolate was identified as Bacillus amyloliquefaciens SS35 based on nucleotide homology and phylogenetic analysis using 16S rDNA and gyrase A gene sequences.