An Effective Procedure for In Vitro Culture of Eleusine coracana (L.) and Its Application. (2nd June 2013)

• Record Type: Journal Article
• Authors: Yemets, Alla I.; Bayer, Galina Ya.; Blume, Yaroslav B.
• Other Names: Berberich T. Academic Editor.; Martin K. P. Academic Editor.; Ogita S. Academic Editor.
• Is Part Of: ISRN botany. Volume 2013(2013)
• Publication Date: 2013-06-02
• Subjects: Botany -- Periodicals; Botany; Periodicals; 580
• DOI: 10.1155/2013/853121
• Languages: English
• ISSNs: 2090-8598

Abstract

Abstract : Efficient protocols for callus production, plantlet regeneration, protoplast isolation, and micronucleation of finger millet ( Eleusine coracana (L.) Gaertn.) were developed. White nodulated calli were formed on medium with N6 macrosalts, MS microsalts, 2.4-dichlorophenoxyacetic acid (2 mg L −1 ), kinetin (0.4 mg L −1 ), 1-naphthalene acetic acid (2 mg L −1 ), and certain additives. It was found that appropriate supplementation leads to formation of numerous shoots. Healthy rooted plantlets formed on hormone-free media. Although different tested additives had no significant effect on percentage of callus formation, it affected callus quality that further dictated plant-forming capacities. Seedlings were better source tissues for protoplasts isolation compared to callus cultures. About 5 × 10 6 protoplasts were isolated from one gram of seedling coleoptyles. Microcolonies were visible after 20–25 days' incubation on KM8p medium supplemented with glutamine (100 mg L −1 ) and proline (500 mg L −1 ). Here we also present a procedure of an efficient induction of micronuclei after chlorpropham (10 μ M) and cytochalasin-B (20 μ M) seedlings treatment with subsequent microprotoplasts isolation. This technique is discussed for the transfer of alien chromosomes and genes from finger millet by microprotoplast-mediated chromosome transfer.