Antiproliferative Action of Conjugated Linoleic Acid on Human MCF-7 Breast Cancer Cells Mediated by Enhancement of Gap Junctional Intercellular Communication through Inactivation of NF-κB. (25th November 2013)

• Record Type: Journal Article
• Authors: Rakib, Md. Abdur; Lee, Won Sup; Kim, Gon Sup; Han, Jae Hee; Kim, Jeong Ok; Ha, Yeong Lae
• Other Names: Chuang Shuang-En Academic Editor.
• Is Part Of: Evidence-based complementary and alternative medicine. Volume 2013(2013)
• Publication Date: 2013-11-25
• Subjects: Alternative medicine -- Periodicals; 615.505
• DOI: 10.1155/2013/429393
• Languages: English
• ISSNs: 1741-427X

Abstract

Abstract : The major conjugated linoleic acid (CLA) isomers, c 9, t 11-CLA and t 10, c 12-CLA, have anticancer effects; however, the exact mechanisms underlying these effects are unknown. Evidence suggests that reversal of reduced gap junctional intercellular communication (GJIC) in cancer cells inhibits cell growth and induces cell death. Hence, we determined that CLA isomers enhance GJIC in human MCF-7 breast cancer cells and investigated the underlying molecular mechanisms. The CLA isomers significantly enhanced GJIC of MCF-7 cells at 40 μ M concentration, whereas CLA inhibited cell growth and induced caspase-dependent apoptosis. CLA increased connexin43 (Cx43) expression both at the transcriptional and translational levels. CLA inhibited nuclear factor- κ B (NF- κ B) activity and enhanced reactive oxygen species (ROS) generation. No significant difference was observed in the efficacy of c 9, t 11-CLA and t 10, c 12-CLA. These results suggest that the anticancer effect of CLA is associated with upregulation of GJIC mediated by enhanced Cx43 expression through inactivation of NF- κ B and generation of ROS in MCF-7 cells.