ECOA-7. Conserved cell-lineage controlled chromatin accessibility in human and mouse glioblastoma stem cells predicts functionally distinct subgroups. (5th July 2021)

• Record Type: Journal Article
• Authors: Lu, Xi; Maturi, Naga Prathyusha; Jarvius, Malin; Zhao, Linxuan; Xie, Yuan; Tan, E-Jean; Xing, Pengwei; Fryknäs, Mårten; Uhrbom, Lene; Chen, Xingqi
• Is Part Of: Neuro-oncology advances. Volume 3(2021)Supplement 2
• Page Start: ii2
• Page End: ii2
• Publication Date: 2021-07-05
• Subjects: 616.99481
• DOI: 10.1093/noajnl/vdab070.007
• Languages: English
• ISSNs: 2632-2498

Abstract

Abstract: There is ample support for developmental control of glioblastoma stem cells (GSCs), and a deeper knowledge of their epigenetic regulation could be central to more efficient glioblastoma (GBM) therapies. For this purpose, we analyzed the chromatin-accessibility landscape of nine mouse GSC cultures of defined cell of origin and 60 patient-derived GSC cultures by assay for transposase-accessible chromatin using sequencing (ATAC-seq). This uncovered an epigenetic variability of both mouse and human GSC cultures that differed from transcriptome clusters. Both mouse and human chromatin accessibility-guided clusters were predominantly determined by distal regulatory elements, displayed unique sets of transcription factor motif enrichment, and exhibited different functional and drug-response properties. Cross-species analysis of distal regulatory element regions in accessible chromatin of mouse and human cultures revealed conserved epigenetic regulation of GSCs.