Integrative analysis of GWAS and transcriptome to reveal novel loci regulation flowering time in semi-winter rapeseed. (September 2021)

• Record Type: Journal Article
• Authors: Huang, Luyao; Min, Yao; Schiessl, Sarah; Xiong, Xinghua; Jan, Habib U.; He, Xin; Qian, Wei; Guan, Chunyun; Snowdon, Rod J.; Hua, Wei; Guan, Mei; Qian, Lunwen
• Is Part Of: Plant science. Volume 310(2021)
• Publication Date: 2021-09
• Subjects: Brassica napus -- Genome-wide association analysis -- Flowering time -- Haplotype -- Coexpression network; Botany -- Periodicals; Botanique -- Périodiques; 580
• DOI: 10.1016/j.plantsci.2021.110980
• Languages: English
• ISSNs: 0168-9452

Abstract

Highlights: GWAS detected twenty-one haplotypes carrying one or more candidate genes significant association with flowering time in rapeseed. To reveal the structural variation of the BnVIN3-C03 gene affection flowering time by PCR amplification and gene expression. BnVIN3-C03 inhibited the expression of BnFLC-A02, BnFLC-A03.1, BnFLC-A10 and BnFLC-C03.1 to regulate the flowering time. Abstract: Flowering is an important turning point from vegetative growth to reproductive growth, and vernalization is an essential condition for the flowering of annual winter plants. To investigate the genetic architecture of flowering time in rapeseed, we used the 60 K Brassica Infinium SNP array to perform a genome-wide analysis of haplotype blocks associated with flowering time in 203 Chinese semi-winter rapeseed inbred lines. Twenty-one haplotype regions carrying one or more candidate genes showed a significant association with flowering time. Interestingly, we detected a SNP (Bn-scaff_22728_1-p285715) located in exon 3 of the BnVIN3-C03 gene that showed a significant association with flowering time on chromosome C03. Based on the SNP alleles A and G, two groups of accessions with early and late flowering time phenotypes were selected, respectively, and PCR amplification and gene expression analysis were combined to reveal the structural variation of the BnVIN3-C03 gene that affected flowering time. Moreover, we found that BnVIN3-C03 inhibited the expression of BnFLC-A02, BnFLC-A0 3 .1, BnFLC-A10 and BnFLC-C03.1, thus modulating the flowering time of Brassica napus. This result provides insight into the genetic improvement of flowering time in B. napus .