VqBGH40a isolated from Chinese wild Vitis quinquangularis degrades trans-piceid and enhances trans-resveratrol. (September 2021)

• Record Type: Journal Article
• Authors: Yan, Chaohui; Yang, Na; Wang, Xinqi; Wang, Yuejin
• Is Part Of: Plant science. Volume 310(2021)
• Publication Date: 2021-09
• Subjects: BGH β-Glycoside hydrolases -- ABA Abscisic acid -- Eth Ethylene -- MeJA Methyl jasmonate -- SA Salicylic acid -- hpi Hours post inoculation -- hpt Hours post treatment -- qRT-PCR Real-time quantitative RT-PCR -- sqRT-PCR Semi-quantitative RT-PCR -- CDS Coding sequence -- IPTG Isopropyl β-d-thiogalactopyranoside -- PBS Phosphate buffer saline -- PMSF Phenylmethylsulfonyl fluoride -- MW Molecular weight -- pI Isoelectric point -- aa Amino acid; β-Glucosidase hydrolase -- Disease resistance -- Grapevine -- Trans-piceid -- Trans-resveratrol; Botany -- Periodicals; Botanique -- Périodiques; 580
• DOI: 10.1016/j.plantsci.2021.110989
• Languages: English
• ISSNs: 0168-9452

Abstract

Highlights: VqBGH40a can hydrolyze trans -piceid and enhance trans -resveratrol content. VqBGH40a involve in the defense mechanism of grapevine against powdery mildew. Grapevine BGH family 1 contains 38 members that are divided into seven groups. Abstract: Resveratrol (3, 5, 4'-trihydroxy-stilbene) is a phytoalexin that can prevent plants from pathogen attacks. Piceid is the glycosylation product of resveratrol and the main storage form of stilbenes in grapevines. Here, we reported the function of a β -glycoside hydrolase gene, VqBGH40a, from the Chinese wild grapevine Vitis quinquangularis accession Danfeng-2 in the regulation of plant resistance to powdery mildew ( Uncinula necator ). VqBGH40a belonging to β -glycoside hydrolase family 1 encoded 506 amino acids and was located on the cytomembrane. Its optimal induction condition was 28 or 30℃, for 4 h, with 0.1 mM IPTG in a prokaryotic expression system. Enzyme activity detection showed that purified VqBGH40a could hydrolyze trans -piceid to form trans -resveratrol in vitro . VqBGH40a was transiently overexpressed in Danfeng-2 leaves and then artificially inoculated with powdery mildew showed that VqBGH40a protein could hydrolyze trans -piceid in vivo . Additionally, a comparative family analysis between VqBGH40a and 38 VviBGHs was performed. Overall, these results demonstrate that VqBGH40a can hydrolyze trans -piceid, enhance trans -resveratrol content, and participate in the defense mechanism of grapevine against powdery mildew.