Dual-chamber differs from single-chamber microbial electrosynthesis in biogas production performance under low temperature (15℃). (October 2021)

• Record Type: Journal Article
• Authors: Wang, Hui; Du, Hongxia; Xie, Haiyin; Zhu, Jiemin; Zeng, Shufang; Igarashi, Yasuo; Luo, Feng
• Is Part Of: Bioresource technology. Volume 337(2021)
• Publication Date: 2021-10
• Subjects: Microbial electrosynthesis -- Methanogenic pathway -- Electroactive microorganisms -- Low temperature -- Metatranscriptomic analysis; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2021.125377
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlight: Acetoclastic pathway was most important in low-temperature MES. Methahnosarcina and Methanothrix were enriched in DC and SC reactor, respectively. Electroactive bacteria Syntrophomonas, Pseudomonas and Bacteroides were dominated. The single-chamber MES achieve acetate to acetyl-CoA by acetate-CoA ligase. The dual-chamber MES was by acetate kinase and phosphate acetyltransferase. Abstract: In this study, single-chamber and dual-chamber Microbial electrosynthesis (MES) with carbon fiber brushes as electrodes were operated at 15°C to compare and analyze the difference in methanogenic performance. Metatranscriptomic analysis showed that the relative abundance of electroactive microorganisms Syntrophomonas, Pseudomonas and Bacteroides in each group exceeded 90%, while the abundance of Geobacter was less than 4%. Acetoclastic methanogens Methahnosarcina was more enriched in dual-chamber MES (61.74%~70.42%), and Methanothrix showed higher abundance in single-chamber MES (33.44%~51.71%). Methahnosarcina and Methanothrix could interact with electroactive microorganisms to improve the electron transfer efficiency through direct interspecies electron transfer (DIET). Analysis of the methane metabolic pathways of low-temperature MES found acetoclastic pathway was domination, and single-chamber MES achieved acetate to acetyl-CoA through acetate-CoA ligase (EC: 6.2.1.1), whereas dual-chamber MES was by acetate kinase (EC: 2.7.2.1) and phosphate acetyltransferase (EC: 2.3.1.8). These results are beneficial to further research on the treatment of low-temperature wastewater.