Influence of culture conditions on ellagitannase expression and fungal ellagitannin degradation. (October 2021)

• Record Type: Journal Article
• Authors: Buenrostro-Figueroa, Juan; Gutierrez-Sánchez, Gerardo; Prado-Barragán, Lilia A.; Rodríguez-Herrera, Raul; Aguilar-Zárate, Pedro; Sepúlveda, Leonardo; Ascacio-Valdés, Juan A.; Tafolla-Arellano, Julio C.; Aguilar, Cristobal N.
• Is Part Of: Bioresource technology. Volume 337(2021)
• Publication Date: 2021-10
• Subjects: Ellagitannase -- Solid-state fermentation -- Protein analysis -- Ellagic acid; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2021.125462
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: PE were used as inducers of EAH by SSF and A. niger GH1. The presence of PE as inducers increased 1142-fold the EAH activity. Three protein groups were identified in both cultures. PLC was overexpressed with PE, showing a great affinity with punicalagin. PLC could act like EAH with an essential role in the ellagitannins biodegradation. Abstract: The role and mechanism of elagitannase is misunderstood because it exhibited different activities due to the low purity or complexity of substrates, and there is no available information about the biochemical, physicochemical and molecular characteristics of the enzyme. This study was aimed to obtain enzymatic extracts by Aspergillus niger GH1 in solid-state fermentation, using dextrose and ellagitannins as inducers of ellagitannase. Protein and bioinformatic analysis were performed to identify the protein sequence expressed in terms of culture conditions. The presence of ellagitannins increased ellagitannase activity 1143-fold compared to dextrose. The higher ellagitannase activity was found at 18 h of culture (1143.30 U g -1 PE). Three groups of proteins were identified in both cultures: β-glucosidase, phospholipase C, and triacylglycerol lipase. However, only phospholipase C was overexpressed with ellagitannins as inducers, showing the most spontaneous reaction with punicalagin (ΔG −8.56). These results suggest that phospholipase could be involved in ellagitannins biosynthesis.