Deficiency of Omentin-1 leads to delayed fracture healing through excessive inflammation and reduced CD31hiEmcnhi vessels. (20th August 2021)

• Record Type: Journal Article
• Authors: Feng, Shi-Kai; Chen, Tuan-Hui; Li, Hong-Ming; Cao, Jia; Liu, Dong-Biao; Rao, Shan-Shan; Liu, Jiang-Hua; Zhang, Yan; Wang, Zhen-Xing; Li, You-You; Tan, Yi-Juan; Liu, Yi-Wei; Hong, Chun-Gu; Yan, Zi-Qi; Chen, Meng-Lu; Wang, Yi-Yi; Yin, Hao; Jin, Ling; Xie, Hui; Wang, Zheng-Guang; Zhou, Yong
• Is Part Of: Molecular and cellular endocrinology. Volume 534(2021)
• Publication Date: 2021-08-20
• Subjects: Fracture healing -- Inflammation -- Omentin-1 -- PDGF-BB -- CD31hiEmcnhi vessel; Endocrinology -- Periodicals; Molecular biology -- Periodicals; Cytology -- Periodicals; Endocrinology -- Periodicals; Hormones -- Periodicals; Endocrinologie -- Périodiques; Cytology; Endocrinology; Molecular biology; Periodicals; 573.4
• DOI: 10.1016/j.mce.2021.111373
• Languages: English
• ISSNs: 0303-7207

Abstract

Abstract: Fracture healing is a complicated process affected by many factors, such as inflammatory responses and angiogenesis. Omentin-1 is an adipokine with anti-inflammatory properties, but whether omentin-1 affects the fracture healing process is still unknown. Here, by using global omentin-1 knockout ( omentin-1 −/− ) mice, we demonstrated that omentin-1 deficiency resulted in delayed fracture healing in mice, accompanied by increased inflammation and osteoclast formation, and decreased production of platelet-derived growth factor-BB (PDGF-BB) and osteogenesis-promoting vessels that are strongly positive for CD31 and Endomucin (CD31 hi Emcn hi ) in the fracture area. In vitro, omentin-1 treatment suppressed the ability of the tumor necrosis factor-α (TNF-α)-activated macrophages to stimulate multi-nuclear osteoclast formation, resulting in a significant increase in the generation of mono-nuclear preosteoclasts and PDGF-BB, a pro-angiogenic protein that is abundantly secreted by preosteoclasts. PDGF-BB significantly augmented endothelial cell proliferation, tube formation and migration, whereas direct treatment with omentin-1 did not induce obvious effects on angiogenesis activities of endothelial cells. Our study suggests a positive role of omentin-1 in fracture healing, which may be associated with the inhibition of inflammation and stimulation of preosteoclast PDGF-BB-mediated promotion of CD31 hi Emcn hi vessel formation. Highlights: Omentin-1 can promote the recovery of mechanical properties during fracture healing. Omentin-1 can promote the formation of CD31 hi Emcn hi vessels during fracture healing. Omentin-1 can promote fracture healing by inhibiting excessive inflammation.