Biophysical and RNA Interference Inhibitory Properties of Oligonucleotides Carrying Tetrathiafulvalene Groups at Terminal Positions. (15th July 2013)

• Record Type: Journal Article
• Authors: Pérez-Rentero, Sónia; Somoza, Alvaro; Grijalvo, Santiago; Janoušek, Jiří; Bělohradský, Martin; Stará, Irena G.; Starý, Ivo; Eritja, Ramon
• Other Names: Benhida Rachid Academic Editor.
• Is Part Of: Journal of chemistry. Volume 2013(2013)
• Publication Date: 2013-07-15
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1155/2013/650610
• Languages: English
• ISSNs: 2090-9063

Abstract

Abstract : Oligonucleotide conjugates carrying a single functionalized tetrathiafulvalene (TTF) unit linked through a threoninol molecule to the 3′ or 5′ ends were synthesized together with their complementary oligonucleotides carrying a TTF, pyrene, or pentafluorophenyl group. TTF-oligonucleotide conjugates formed duplexes with higher thermal stability than the corresponding unmodified oligonucleotides and pyrene- and pentafluorophenyl-modified oligonucleotides. TTF-modified oligonucleotides are able to bind to citrate-stabilized gold nanoparticles (AuNPs) and produce stable gold AuNPs functionalized with oligonucleotides. Finally, TTF-oligoribonucleotides have been synthesized to produce siRNA duplexes carrying TTF units. The presence of the TTF molecule is compatible with the RNA interference mechanism for gene inhibition.