Automated improvement of stickleback reference genome assemblies with Lep‐Anchor software. (12th May 2021)

• Record Type: Journal Article
• Authors: Kivikoski, Mikko; Rastas, Pasi; Löytynoja, Ari; Merilä, Juha
• Is Part Of: Molecular ecology resources. Volume 21:Number 6(2021)
• Page Start: 2166
• Page End: 2176
• Publication Date: 2021-05-12
• Subjects: Gasterosteus -- genome assembly -- haplotype -- mosaicism -- Pungitius -- stickleback; Molecular ecology -- Periodicals; 572.8
• DOI: 10.1111/1755-0998.13404
• Languages: English
• ISSNs: 1755-098X

Abstract

Abstract: We describe an integrative approach to improve contiguity and haploidy of a reference genome assembly and demonstrate its impact with practical examples. With two novel features of Lep‐Anchor software and a combination of dense linkage maps, overlap detection and bridging long reads, we generated an improved assembly of the nine‐spined stickleback ( Pungitius pungitius ) reference genome. We were able to remove a significant number of haplotypic contigs, detect more genetic variation and improve the contiguity of the genome, especially that of X chromosome. However, improved scaffolding cannot correct for mosaicism of erroneously assembled contigs, demonstrated by a de novo assembly of a 1.6‐Mbp inversion. Qualitatively similar gains were obtained with the genome of three‐spined stickleback ( Gasterosteus aculeatus ). Since the utility of genome‐wide sequencing data in biological research depends heavily on the quality of the reference genome, the improved and fully automated approach described here should be helpful in refining reference genome assemblies.