Inflammatory profile of apical periodontitis associated with liver fibrosis in rats: histological and immunohistochemical analysis. (4th April 2021)

• Record Type: Journal Article
• Authors: Cantiga‐Silva, C.; Estrela, C.; Segura‐Egea, J. J.; Azevedo, J. P.; de Oliveira, P. H. C.; Cardoso, C. B. M.; Pinheiro, T. N.; Ervolino, E.; Sivieri‐Araújo, G.; Cintra, L. T. A.
• Is Part Of: International endontic journal. Volume 54:Number 8(2021)
• Page Start: 1353
• Page End: 1361
• Publication Date: 2021-04-04
• Subjects: apical periodontitis -- cytokines -- inflammation -- liver fibrosis -- oral infection; Endodontics -- Periodicals; 617.6342
• DOI: 10.1111/iej.13519
• Languages: English
• ISSNs: 0143-2885

Abstract

Abstract: Aim: To investigate the effects of liver fibrosis (LF) on the pro‐inflammatory mediators and periapical bone resorption of apical periodontitis (AP) in rats. Methodology: Forty male Wistar rats were distributed into four groups: C – control, AP – rats with AP, LF – rats with LF, AP + LF – rats with AP and LF. LF was induced by carbon tetrachloride administration for 8 weeks and surgical bile duct ligation for 4 weeks; AP was induced in the teeth of rats by dental pulp exposure to the oral environment for 30 days. Jaws and livers were removed after euthanasia. Haematoxylin and Eosin (H&E) and Picrosirius Red (PSR) staining were used to confirm fibrosis in the livers. The jaws were analysed using H&E staining, immunohistochemical assays of interleukin (IL)‐1β, IL‐6 and tumour necrosis factor‐alpha (TNF‐α). Student's t ‐test and Mann–Whitney's U ‐test were used for statistical analysis ( P < 0.05). Results: Inflammatory infiltrate was moderate in the AP group and severe in the AP + LF group ( P < 0.05). Periapical bone resorption was significantly larger in the AP + LF group compared with the AP group ( P < 0.05). IL‐1β, IL‐6 and TNF‐α levels were significantly higher in AP + LF group when compared to the AP group ( P < 0.05). Conclusion: More intense inflammatory infiltrate, greater amounts of pro‐inflammatory cytokines and increased periapical bone resorption were observed in the presence of liver fibrosis in rats with exposed pulps.