Fine structure analysis of perineuronal nets in the ketamine model of schizophrenia. (25th June 2020)

• Record Type: Journal Article
• Authors: Kaushik, Rahul; Lipachev, Nikita; Matuszko, Gabriela; Kochneva, Anastasia; Dvoeglazova, Anastasia; Becker, Axel; Paveliev, Mikhail; Dityatev, Alexander
• Other Names: Dityatev Alexander guestEditor.; Seidenbecher Constanze guestEditor.; Morawski Markus guestEditor.
• Is Part Of: European journal of neuroscience. Volume 53:Number 12(2021)
• Page Start: 3988
• Page End: 4004
• Publication Date: 2020-06-25
• Subjects: chondroitin sulfate proteoglycans -- extracellular matrix -- parvalbumin -- Wisteria floribunda agglutinin -- synapses; Nervous system -- Periodicals; 612.8
• DOI: 10.1111/ejn.14853
• Languages: English
• ISSNs: 0953-816X

Abstract

Abstract: Perineuronal nets (PNNs) represent a highly condensed specialized form of brain extracellular matrix (ECM) enwrapping mostly parvalbumin‐positive interneurons in the brain in a mesh‐like fashion. PNNs not only regulate the onset and completion of the critical period during postnatal brain development, control cell excitability, and synaptic transmission but are also implicated in several brain disorders including schizophrenia. Holes in the perineuronal nets, harboring the synaptic contacts, along with hole‐surrounding ECM barrier can be viewed as PNN compartmentalization units that might determine the properties of synapses and heterosynaptic communication. In this study, we developed a novel open‐source script for Fiji (ImageJ) to semi‐automatically quantify structural alterations of PNNs such as the number of PNN units, area, mean intensity of PNN marker expression in 2D and 3D, shape parameters of PNN units in the ketamine‐treated Sprague–Dawley rat model of schizophrenia using high‐resolution confocal microscopic images. We discovered that the mean intensity of ECM within PNN units is inversely correlated with the area and the perimeter of the PNN holes. The intensity, size, and shape of PNN units proved to be three major principal factors to describe their variability. Ketamine‐treated rats had more numerous but smaller and less circular PNN units than control rats. These parameters allowed to correctly classify individual PNNs as derived from control or ketamine‐treated groups with ≈85% reliability. Thus, the proposed multidimensional analysis of PNN units provided a robust and comprehensive morphometric fingerprinting of fine ECM structure abnormalities in the experimental model of schizophrenia. Abstract : The neural extracellular matrix plays an important role in shaping synapses in healthy and diseased brains but the methods for quantitative analysis of its fine organization are just emerging. Here, we developed a tool for semi‐automatic decomposition of perineuronal nets into a set of perisomatic circular‐like units enriched in the perisynaptic extracellular matrix. We used this tool to reveal remarkable alterations in the number, size, and shape of these units in the ketamine model of schizophrenia.