Lactic acid bacteria biofilms and their ability to mitigate Escherichia coli O157:H7 surface colonization. (3rd June 2021)

• Record Type: Journal Article
• Authors: Cisneros, L.; Cattelan, N.; Villalba, M.I.; Rodriguez, C.; Serra, D.O.; Yantorno, O.; Fadda, S.
• Is Part Of: Letters in applied microbiology. Volume 73:Number 2(2021)
• Page Start: 247
• Page End: 256
• Publication Date: 2021-06-03
• Subjects: biocontrol -- biofilm -- Escherichia coli O157:H7 -- lactic acid bacteria -- surface colonization; Microbiology -- Periodicals; 660.62
• DOI: 10.1111/lam.13509
• Languages: English
• ISSNs: 0266-8254

Abstract

Abstract: Lactic acid bacteria (LAB) exert antagonistic activities against diverse microorganisms, including pathogens. In this work, we aimed to investigate the ability of LAB strains isolated from food to produce biofilms and to inhibit growth and surface colonization of Enterohaemorrhagic Escherichia coli (EHEC) O157:H7 at 10°C. The ability of 100 isolated LAB to inhibit EHEC O157:H7 NCTC12900 growth was evaluated in agar diffusion assays. Thirty‐seven LAB strains showed strong growth inhibitory effect on EHEC. The highest inhibitory activities corresponded to LAB strains belonging to Lactiplantibacillus plantarum, Pediococcus acidilactici and Pediococcus pentosaceus species. Eighteen out of the 37 strains that showed growth inhibitory effects on EHEC also had the ability to form biofilms on polystyrene surfaces at 10°C and 30°C. Pre‐established biofilms on polystyrene of four of these LAB strains were able to reduce significantly surface colonization by EHEC at low temperature (10°C). Among these four strains, Lact. plantarum CRL 1075 not only inhibited EHEC but also was able to grow in the presence of the enteric pathogen. Therefore, this strain proved to be a good candidate for further technological studies oriented to its application in food‐processing environments to mitigate undesirable surface contaminations of E. coli . Abstract : Significance and Impact of the Study: Nowadays, the use of LAB (lactic acid bacteria) in food‐processing environments is considered as a biological strategy to control food‐borne pathogens. This work provides new insights about the capacity of LAB to form biofilms and to inhibit growth and surface colonization of Enterohaemorrhagic Escherichia coli (EHEC) O157:H7 under usual meat‐processing environments. Our findings support the use of biofilm‐forming LAB strains as a biological strategy to control EHEC contaminations from food‐processing surfaces.