Biosynthesis of [14C]‐11‐De‐O‐Methyltomaymycin, a Precursor of Radiolabelled Antibody Drug Conjugates. (1st June 2021)

• Record Type: Journal Article
• Authors: Aubert, Catherine; Lavisse, Mélanie; Roy, Sebastien
• Is Part Of: Chembiochem. Volume 22:Number 14(2021)
• Page Start: 2424
• Page End: 2429
• Publication Date: 2021-06-01
• Subjects: biosynthesis -- 14C-L-tyrosine -- 14C-anthranilic acid -- de-O-methyltomaymycin -- Streptomyces; Biochemistry -- Periodicals; Molecular biology -- Periodicals; Pharmaceutical chemistry -- Periodicals; 572
• DOI: 10.1002/cbic.202100080
• Languages: English
• ISSNs: 1439-4227

Abstract

Abstract: Antibody drug conjugates (ADCs) are one of the most promising technologies to treat cancer as they combine the specificity of an antibody with the high potency of a cytotoxic molecule such as tomaymycin derivatives, which are DNA‐interactive antitumor antibiotics previously isolated from bacterial broth. The multistep chemical synthesis of some tomaymycin derivatives is complicated because their structures contain a reactive imine bond. Therefore, we turned to biosynthesis to obtain 14 C radiolabelled tomaymycin derivative to support ADME studies. Following Hurley's work ( J. Antibiotics 1977, 30, 349–370; Antimicrob. Agents Chemother . 1979, 15, 42–45; Acc. Chem. Res . 1980, 13, 263–269), the 14 C radiolabel was incorporated efficiently in one step from radiolabelled tyrosine using the strain Streptomyces sp. FH6421. This process has been further optimized by using anthranilic acid as radiolabelled precursor, leading to one of the highest incorporation levels of radiochemical precursors published to date. This biosynthetic strategy is the fastest way to access such radiolabelled precursors. Abstract : The aim of this research was to avoid a radiolabelled multistep chemical synthesis of tomaymycin derivative using biosynthesis. The 14 C radiolabel was incorporated efficiently in one step from radiolabelled tyrosine using the strain Streptomyces sp. FH6421. Optimization by using radiolabelled anthranilic acid led to one of the highest incorporation levels of radiochemical precursors published to date.