Bioinorganic Synthesis of Polyrhodanine Stabilized Fe3O4/Graphene Oxide in Microbial Supernatant Media for Anticancer and Antibacterial Applications. (28th June 2021)

• Record Type: Journal Article
• Authors: Mousavi, Seyyed Mojtaba; Hashemi, Seyyed Alireza; Gholami, Ahmad; Omidifar, Navid; Zarei, Maryam; Bahrani, Sonia; Yousefi, Khadije; Chiang, Wei-Hung; Babapoor, Aziz
• Other Names: Tan Songwen Academic Editor.
• Is Part Of: Bioinorganic chemistry and applications. Volume 2021(2021)
• Publication Date: 2021-06-28
• Subjects: Bioinorganic chemistry -- Periodicals; Bioinorganic chemistry; Biochemistry; Inorganic Chemistry; Chemistry, Bioinorganic; Periodicals; 572.51
• DOI: 10.1155/2021/9972664
• Languages: English
• ISSNs: 1565-3633

Abstract

Abstract : Polyrhodanines have been broadly utilized in diverse fields due to their attractive features. The effect of polyrhodanine- (PR-) based materials on human cells can be considered a controversial matter, while many contradictions exist. In this study, we focused on the synthesis of polyrhodanine/Fe3 O4 modified by graphene oxide and the effect of kombucha (Ko) supernatant on results. The general structure of synthetic compounds was determined in detail through Fourier-transform infrared spectroscopy (FT-IR). Also, obtained compounds were morphologically, magnetically, and chemically characterized using scanning electron microscopy (SEM) and vibrating sample magnetometer (VSM), energy dispersive X-ray (EDX) analysis. The antibacterial effects of all synthesized nanomaterials were done according to CLSI against four infamous pathogens. Also, the cytotoxic effects of the synthesized compounds on the human liver cancer cell line (Hep-G2) were assessed by MTT assay. Our results showed that Go/Fe has the highest average inhibitory effect against Escherichia coli and Pseudomonas aeruginosa, and this compound possesses the least antimicrobial effect on Staphylococcus aureus . Considering the viability percent of cells in the PR/GO/Fe3 O4 compound and comparing it with GO/Fe3 O4, it can be understood that the toxic effects of polyrhodanine can diminish the metabolic activity of cells at higher concentrations (mostly more than 50 µ g/mL), and PR/Fe3 O4 /Ko exhibited some promotive effects on cell growth, which enhanced the viability percent to more than 100%. Similarly, the cell viability percent of PR/GO/Fe3 O4 /KO compared to PR/GO/Fe3 O4 is much higher, which can be attributed to the presence of kombucha in the compound. Consequently, based on the results, it can be concluded that this novel polyrhodanine-based nanocompound can act as drug carriers due to their low toxic effects and may open a new window on the antibacterial agents.