Stabilisation of the Fatty Acid Decarboxylase from Chlorella variabilis by Caprylic Acid. (1st June 2021)

• Record Type: Journal Article
• Authors: Wu, Yinqi; Paul, Caroline E.; Hollmann, Frank
• Is Part Of: Chembiochem. Volume 22:Number 14(2021)
• Page Start: 2420
• Page End: 2423
• Publication Date: 2021-06-01
• Subjects: caprylic acid -- fatty acids -- fatty acid photodecarboxylase -- photochemical inactivation -- photostability; Biochemistry -- Periodicals; Molecular biology -- Periodicals; Pharmaceutical chemistry -- Periodicals; 572
• DOI: 10.1002/cbic.202100182
• Languages: English
• ISSNs: 1439-4227

Abstract

Abstract: The fatty acid photodecarboxylase from Chlorella variabilis NC64 A ( Cv FAP) catalyses the light‐dependent decarboxylation of fatty acids. Photoinactivation of Cv FAP still represents one of the major limitations of this interesting enzyme en route to practical application. In this study we demonstrate that the photostability of Cv FAP can easily be improved by the administration of medium‐chain length carboxylic acids such as caprylic acid indicating that the best way of maintaining Cv FAP stability is 'to keep the enzyme busy'. Abstract : Keep me busy : The photoactivated fatty acid decarboxylase from Chlorella variabilis NC64 A ( Cv FAP) is a promising catalyst to generate next generation biodiesel from (waste) fatty acids. CvFAP, however is also plagued by pronounced photoinactivation, which can be alleviated by keeping it busy, i. e. converting carboxylic acids such as caprylic acid.