Corni Fructus Containing Formulation Attenuates Weight Gain in Mice with Diet-Induced Obesity and Regulates Adipogenesis through AMPK. (19th September 2013)

• Record Type: Journal Article
• Authors: Kim, Hye-Lin; Jeon, Yong-Deok; Park, Jinbong; Rim, Hong-Kun; Jeong, Mi-Young; Lim, Hara; Ko, Seong-Gyu; Jang, Hyeung-Jin; Lee, Byung-Cheol; Lee, Kyung-Tae; Lee, Kang-Min; Lee, Hyejung; Kim, Sung-Hoon; Kim, Su-Jin; Hong, Seung-Heon; Um, Jae-Young
• Other Names: Jang Bo-Hyoung Academic Editor.
• Is Part Of: Evidence-based complementary and alternative medicine. Volume 2013(2013)
• Publication Date: 2013-09-19
• Subjects: Alternative medicine -- Periodicals; 615.505
• DOI: 10.1155/2013/423741
• Languages: English
• ISSNs: 1741-427X

Abstract

Abstract : Obesity is a metabolic disorder characterized by chronic inflammation and dyslipidemia and is a strong predictor for the development of hypertension, diabetes mellitus, and cardiovascular disease. This study examined the antiobesity effect of an ethanol extract of Corni Fructus containing formulation (CDAP), which is a combination of four natural components: Corni Fructus, Dioscoreae Rhizoma, Aurantii Fructus Immaturus, and Platycodonis Radix. The cellular lipid content in 3T3-L1 adipocytes was assessed by Oil Red O staining. Expressions of peroxisome proliferator-activated receptor- γ (PPAR- γ ), CCAAT/enhancer-binding protein- α (C/EBP- α ), and lipin-1 were determined by real-time RT-PCR. Western blot was used to determine the protein levels of PPAR- γ, C/EBP- α, and AMP-activated protein kinase- α (AMPK- α ). The CDAP extract suppressed the differentiation of 3T3-L1 adipocytes by downregulating cellular induction of PPAR- γ, C/EBP- α, and lipin-1. The CDAP extract also significantly upregulated phosphorylation of AMPK- α . An in vivo study showed that CDAP induced weight loss in mice with high-fat-diet-induced obesity. These results indicate that CDAP has a potent anti-obesity effect due to the inhibition of adipocyte differentiation and adipogenesis.