The Vacuolar-Type H+-ATPase in Ovine Rumen Epithelium is Regulated by Metabolic Signals. (4th January 2010)

• Record Type: Journal Article
• Authors: Kuzinski, Judith; Zitnan, Rudolf; Warnke-Gurgel, Christina; Schweigel, Monika
• Other Names: Kitamura Kenichiro Academic Editor.
• Is Part Of: Journal of biomedicine and biotechnology. Volume 2010(2010)
• Publication Date: 2010-01-04
• Subjects: Medicine -- Periodicals; Biology -- Periodicals; Biotechnology -- Periodicals; Medicine; Biology; Biotechnology; Médecine; Biologie; Biotechnologie; Biology; Biotechnology; Medicine; Biotechnology; Biomedicine; Electronic journals; Periodical; Periodicals; Electronic journals; 610
• DOI: 10.1155/2010/525034
• Languages: English
• ISSNs: 1110-7243

Abstract

Abstract : In this study, the effect of metabolic inhibition (MI) by glucose substitution with 2-deoxyglucose (2-DOG) and/or application of antimycin A on ovine rumen epithelial cells (REC) vacuolar-type H + -ATPase (vH + -ATPase) activity was investigated. Using fluorescent spectroscopy, basal pH i of REC was measured to be 7.3 ± 0.1 in HCO 3 − -free, glucose-containing NaCl medium. MI induced a strong pH i reduction (− 0.44 ± 0.04 pH units) with a more pronounced effect of 2-DOG compared to antimycin A (− 0.30 ± 0.03 versus − 0.21 ± 0.03 pH units). Treatment with foliomycin, a specific vH + -ATPase inhibitor, decreased REC pH i by 0.21 ± 0.05 pH units. After MI induction, this effect was nearly abolished (− 0.03 ± 0.02 pH units). In addition, membrane-associated localization of vH + -ATPase B subunit disappeared. Metabolic control of vH + -ATPase involving regulation of its assembly state by elements of the glycolytic pathway could provide a means to adapt REC ATP consumption according to energy availability.