Β-Glucan Oligosaccharide Enhances CD8+ T Cells Immune Response Induced by a DNA Vaccine Encoding Hepatitis B Virus Core Antigen. (10th June 2010)

• Record Type: Journal Article
• Authors: Wang, Jing; Dong, Shengfu; Liu, Chunhong; Wang, Wei; Sun, Shuhui; Gu, Jianxin; Wang, Yuan; Boraschi, Diana; Qu, Di
• Other Names: Xiao Zhengguo Academic Editor.
• Is Part Of: Journal of biomedicine and biotechnology. Volume 2010(2010)
• Publication Date: 2010-06-10
• Subjects: Medicine -- Periodicals; Biology -- Periodicals; Biotechnology -- Periodicals; Medicine; Biology; Biotechnology; Médecine; Biologie; Biotechnologie; Biology; Biotechnology; Medicine; Biotechnology; Biomedicine; Electronic journals; Periodical; Periodicals; Electronic journals; 610
• DOI: 10.1155/2010/645213
• Languages: English
• ISSNs: 1110-7243

Abstract

Abstract : DNA vaccination can induce specific CD 8 + T cell immune response, but the response level is low in large mammals and human beings. Coadministration of an adjuvant can optimize protective immunity elicited by a DNA vaccine. In this study, we investigated the effect of a synthetic glucohexaose (β -glu6), an analogue of Lentinan basic unit, on specific CD 8 + T cell response induced by a DNA vaccine encoding HBcAg (pB144) in mice. We found that β -glu6 promoted the recruitment and maturation of dendritic cells, enhanced the activation of CD 8 + and CD 4 + T cells and increased the number of specific CD 8 + /IFN-γ + T cells in lymphoid and nonlymphoid tissues in mice immunized by pB144. Immunization with pB144 and β -glu6 increased the anti-HBc IgG and IgG2a antibody titer. These results demonstrate that β -glu6 can enhance the virus-specific CTL and Th1 responses induced by DNA vaccine, suggesting β -glu6 as a candidate adjuvant in DNA vaccination.