A ligation-driven CRISPR–Cas biosensing platform for non-nucleic acid target detections. Issue 57 (28th June 2021)

• Record Type: Journal Article
• Authors: Zhao, Jiali; Tan, Zhen; Wang, Liu; Lei, Chunyang; Nie, Zhou
• Is Part Of: Chemical communications. Volume 57:Issue 57(2021)
• Page Start: 7051
• Page End: 7054
• Publication Date: 2021-06-28
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/d1cc02578c
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : A ligation-driven CRISPR– Cas biosensing platform was proposed to detect different non-nucleic acid targets with high sensitivity and specificity. Abstract : Herein, we describe a CRISPR– Cas12a sensing platform activated by a DNA ligation reaction for the sensitive detection of non-nucleic acid targets, including NAD +, ATP and polynucleotide kinase (PNK). In this design, the DNA ligation reaction triggered by these biomolecules generates DNA duplexes, which can activate the nuclease activity of Cas12a to produce amplified fluorescence signals. As a result, this work provides an alternative strategy to expand the applicability of the CRISPR– Cas system into the detection of non-nucleic acid biomolecules.