Salipiger mangrovisoli sp. nov., isolated from mangrove soil and the proposal for the reclassification of Paraphaeobacter pallidus as Salipiger pallidus comb. nov. Issue 7 (16th July 2021)

• Record Type: Journal Article
• Authors: Du, Juan; Liu, Yang; Pei, Tao; Deng, Ming-Rong; Zhu, Honghui
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 71:Issue 7(2021)
• Publication Date: 2021-07-16
• Subjects: genotypes and phenotypes -- polyphasic taxonomy -- reclassification -- Salipiger mangrovisoli; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.004892
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : A novel Gram-stain-negative, aerobic and rod-shaped bacterial strain designated as 6D45A T was isolated from mangrove soil and characterized using a polyphasic taxonomic approach. Strain 6D45A T was found to grow at 10–37 °C (optimum, 28 °C), at pH 6.0–9.0 (optimum, 7.0) and in 0–5 % (w/v) NaCl (optimum, 2%). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 6D45A T fell into the genus Salipiger and shared 99.1 % identity with the closest type strain Salipiger pacificus CGMCC 1.3455 T and less than 97.2 % identity with other type strains of this genus. The 34.8 % digital DNA–DNA hybridization (dDDH) and 88.3 % average nucleotide identity (ANI) values between strain 6D45A T and the closest relative above were well below recognized thresholds of 70 % DDH and 95–96 % ANI for species definition, implying that strain 6D45A T should represent a novel genospecies. The phylogenomic analysis indicated that strain 6D45A T formed an independent branch distinct from reference strains. The predominant cellular fatty acid of strain 6D45A T was summed feature 8 (C18 : 1 ω6 c and/or C18 : 1 ω7 c, 66.9 %); the polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, two unidentified aminolipids, two unidentified glycolipids and an unknown lipid; the respiratory quinone was Q-10. The genomic DNA G+C content was 66.5 mol %. Based on the phenotypic and genotypic characteristics, strain 6D45A T is concluded to represent a novel species of the genus Salipiger, for which the name Salipiger mangrovisoli sp. nov., is proposed. The type strain of the species is 6D45A T (=GDMCC 1.1960 T =KCTC 82334 T ). We also propose the reclassification of Paraphaeobacter pallidus as Salipiger pallidus comb. nov. and ' Pelagibaca abyssi ' as a species of the genus Salipiger .