Immunological response of fallopian tube epithelial cells to spermatozoa through modulating cytokines and chemokines. (August 2021)

• Record Type: Journal Article
• Authors: Mousavi, Seyed Omidreza; Mohammadi, Roudabeh; Amjadi, Fatemehsadat; Zandieh, Zahra; Aghajanpour, Samaneh; Aflatoonian, Khashayar; Sabbaghian, Marjan; Eslami, Maryam; Madani, Tahereh; Aflatoonian, Reza
• Is Part Of: Journal of reproductive immunology. Volume 146(2021)
• Publication Date: 2021-08
• Subjects: Fallopian tube -- Epithelial cell -- Spermatozoa -- Cytokine -- QPCR array; Reproduction -- Immunological aspects -- Periodicals; Immunology -- Periodicals; Allergy and Immunology -- Periodicals; Reproduction -- Periodicals; Reproduction -- Immunologie -- Périodiques; Immunologie -- Périodiques; Immunology; Reproduction -- Immunological aspects; Periodicals; Electronic journals; Electronic journals; 615.766
• DOI: 10.1016/j.jri.2021.103327
• Languages: English
• ISSNs: 0165-0378

Abstract

Highlights: Sperms-fallopian tubes Interaction plays a significant role in fertilization. Human cell line of fallopian tubes was co-incubated to investigate the interaction. 84 Cytokine's expression was evaluated by PCR array. Spermatozoa downregulates expression of inflammatory and chemotactic cytokines. Altered genes' expression were toward immunological tolerance to spermatozoa. Abstract: Background: Spermatozoa interactions with fallopian tubes may influence fertilization. The purpose was to investigate cytokines, chemokines and growth factors expression from human fallopian tube epithelial cells (OE-E6/E7) exposed to spermatozoa. Methods: Fresh semen samples were obtained from 10 healthy normozoospermic men. Sperms were prepared and co-cultured with OE-E6/E7. The cell line without spermatozoa was considered as the control group. Afterwards, Expression of 84 cytokines from OE-E6/E7 cell line in the presence and absence of spermatozoa were measured using PCR-array. Quantitative PCR was performed on seven genes to confirm the results of PCR-array analysis. Differentially expressed genes were subjected to www.geneontology.org and www.pantherdb.org to perform GO enrichment and panther pathway analysis. The concentration of IL-8, IL- 10, IL-1B and BMP-4 in culture medium were analyzed by ELISA. Results: Sperm interaction with the epithelial cells resulted in a significant increase in expression of TGF-β2, BMP-4, IL-10, IL-9, and CD40LG markers. Moreover, expression of IL-16, IL-17F, SPP-1, CXCL-13, MSTN, IL-1A, IL-1B, IL-8, BMP-7, CSF-2, CSF-3, VEGF-A, OSM, LTA, TNF, TNFRSF11B, TNFSF11, CCL-11, CCL-20, CCL-24, CCL-3, CCL-8, CX3CL1 and CXCL-9 were considerably reduced in presence of spermatozoa. Panther pathway analysis discovered 3 pathways for upregulated genes including gonadotropin-releasing hormone receptor, TGF-beta and interleukin signaling pathways. Furthermore, 9 pathways were detected for down-regulated genes. Inflammation signaling pathway which is mediated by chemokine and cytokine contains the most number of genes. Conclusion: This study indicates that sperm modifies expression of cytokines, chemokines and growth factors from OE-E6/E7. Moreover, altered genes expression are toward higher survival chance of the spermatozoa.