Fluorescence enhancement of rhodamine B and fluorescein immobilized on optimized ZrO2 nano-porous films. (October 2021)

• Record Type: Journal Article
• Authors: Zhu, Miao; Wang, Xixin; Liu, Dongxin; Yin, Zekun; Li, Fenglin; Zhao, Jianling
• Is Part Of: Journal of physics and chemistry of solids. Volume 157(2021)
• Publication Date: 2021-10
• Subjects: ZrO2 films -- Rhodamine B -- Fluorescein -- FRET -- Fluorescence performance; Solids -- Periodicals; Solides -- Périodiques; Solids; Periodicals; 530.41
• DOI: 10.1016/j.jpcs.2021.110198
• Languages: English
• ISSNs: 0022-3697

Abstract

Abstract: ZrO2 nano-porous films with different morphologies were prepared by anodic oxidation method, and then rhodamine B (RB) and fluorescein (FL) were immobilized on the surface of ZrO2 to obtain composite fluorescent films. The effects of ZrO2 morphology, loading amount of RB and FL, excitation wavelength, and FL/RB ratio on the fluorescence performance of the composite films and the FL-RB fluorescence resonance energy transfer (FRET) system were studied in detail. The related reasons were investigated as well. Results show that the ZrO2 film with rough surface and tiny pores is more favorable for fluorescence emission. In the FRET system, the energy transfer efficiency of FL and the actual enhancement effect on RB vary with the FL/RB ratio and the loading amount. Moreover, the fluorescence enhancement effect of FL on RB is also closely related to the excitation wavelength. Compared with that in aqueous solutions, immobilization on the optimized ZrO2 nano-porous film can dramatically improve the fluorescence performance of RB and FL, making their emission intensity increased by 4.5 and 5.7 times, respectively. The efficiency of FRET system on ZrO2 nano-porous film can reach more than 73.2%, which is significantly higher than that in aqueous solution (23.6%). Graphical abstract: Image 1 Highlights: ZrO2 films with different morphologies were prepared by anodic oxidation. FL and RB were immobilized on ZrO2 to prepare composite fluorescent films. The emission intensities of RB and FL are increased by 4–6 times by ZrO2 film. The FRET efficiency of FL-RB on ZrO2 film can reach 73.2%. The maximum fluorescence enhancement amplitude of RB is 7.3 times on ZrO2 film.