Development of endodontic sealers containing antimicrobial-loaded polymer particles with long-term antibacterial effects. Issue 8 (August 2021)

• Record Type: Journal Article
• Authors: Kitagawa, Haruaki; Kitagawa, Ranna; Tsuboi, Ririko; Hirose, Nanako; Thongthai, Pasiree; Sakai, Hirohiko; Ueda, Mayuka; Ono, Shunka; Sasaki, Jun-Ichi; Ooya, Tooru; Imazato, Satoshi
• Is Part Of: Dental materials. Volume 37:Issue 8(2021)
• Page Start: 1248
• Page End: 1259
• Publication Date: 2021-08
• Subjects: polyHEMA/TMPT polyhydroxyethyl methacrylate trimethylolpropane trimethacrylate -- CPC cetylpyridinium chloride -- CLP CPC-loaded polyHEMA/TMPT particles -- HEMA 2-hydroxyethyl methacrylate -- TMPT trimethylolpropane trimethacrylate -- FGF-2 fibroblast growth factor-2 -- 10%-CLP 10 wt.% CPC-loaded polyHEMA/TMPT particle -- 25%-CLP 25 wt.% CPC-loaded polyHEMA/TMPT particle -- s small size -- m middle size -- ℓ large size -- HPLC high-performance liquid chromatography -- NaOCl sodium hypochlorite -- EDTA ethylenediaminetetraacetic acid -- SEM scanning electron microscope -- BHI brain heart infusion -- B&B Brown and Brenn -- CLSM confocal laser scanning microscope -- MC3T3-E1 osteoblastic cell line -- MTT 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide -- MEM minimum essential medium -- ANOVA analysis of variance -- HSD honestly significant difference -- MIC minimum inhibitory concentration -- 4-META 4-methacryloyloxyethyl trimellitate anhydride; PolyHEMA/TMPT particle -- Antimicrobial -- Infection control -- Drug delivery -- Endodontic filling -- Resin -- Cetylpyridinium chloride -- Antibacterial -- Sealer -- Sustained release; Dentistry -- Periodicals; Dental materials -- Periodicals; 617.695
• DOI: 10.1016/j.dental.2021.04.008
• Languages: English
• ISSNs: 0109-5641

Abstract

Highlights: Novel CPC-loaded particles were incorporated into the HEMA-based endodontic sealer. Sustained CPC release from the experimental sealer was observed. Residual bacteria in root canals were eradicated by obturation with the sealer. Long-term antibacterial activities were achieved by incorporation of the particles. Incorporation of particles had no influence on physical properties of the sealer. Abstract: Objective: The objective of this study is to prepare new dental resins with a long-lasting antimicrobial activity. Specifically, this study evaluates an approach for controlling infection in root canals using sealers containing polyhydroxyethyl methacrylate trimethylolpropane trimethacrylate (polyHEMA/TMPT) particles loaded with cetylpyridinium chloride (CPC). In addition, the physical properties of sealers containing CPC-loaded polyHEMA/TMPT particles (CLP) are determined. Methods: PolyHEMA/TMPT particles with 10 (10%-CLP) and 25 wt.% CPC (25%-CLP) with different particle sizes were fabricated and incorporated in HEMA-based sealers. CPC-release profiles were evaluated over 14 days of immersion in water, followed by 14 days of storage and 14 days of water immersion. The antibacterial activity of these sealers against Enterococcus faecalis in dentinal tubules was assessed using a root-canal-infection model. Their sealing abilities were evaluated by fluid filtration and physical properties were tested according to the ISO 6876 standard. The long-term antibacterial activity of the cured sealer containing 25%-CLP (∼21 μm particle diameter) was re-assessed after 1 year of storage. Results: After 28 days of immersion, 25%-CLP exhibited a higher and sustained CPC release unlike 10%-CLP. Residual bacteria in root dentinal tubules were eradicated by obturation with 25%-CLP-containing sealers. The incorporation of 25%-CLP (∼21 μm) had no adverse effects on the sealing ability and physical properties of the sealer and resulted in long-term antibacterial activity. Significance: The incorporation of CPC-loaded particles in HEMA resins yielded endodontic sealers with long-term bactericidal activity against E. faecalis in root canals. These sealers can potentially be used to prevent recurrent apical periodontitis.