Evaluating CO2 emissions from continuous flow and batch growth systems under autotrophic mode: Implications for GHG accounting of biological nutrient removal. (15th September 2021)

• Record Type: Journal Article
• Authors: Ronan, Evan; Kroukamp, Otini; Liss, Steven N.; Wolfaardt, Gideon
• Is Part Of: Journal of environmental management. Volume 294(2021)
• Publication Date: 2021-09-15
• Subjects: Autotrophic ammonia oxidation -- Autotrophic biofilms -- Biological nutrient removal -- Carbonate alkalinity -- CO2 emissions -- Greenhouse gas emissions; Environmental policy -- Periodicals; Environmental management -- Periodicals; Environment -- Periodicals; Ecology -- Periodicals; 363.705
• DOI: 10.1016/j.jenvman.2021.112928
• Languages: English
• ISSNs: 0301-4797

Abstract

Abstract: The oxidation of ammonia by autotrophic bacteria is a central part of the nitrogen cycle and a fundamental aspect of biological nutrient removal (BNR) during wastewater treatment. Autotrophic ammonia oxidation produces protons and results in net-CO2 production due to the neutralizing effect of bicarbonate alkalinity. Attention must be paid to the propensity for this produced CO2 to be transferred to the atmosphere where it can act as a greenhouse gas (GHG). In the context of BNR systems, bicarbonate-derived CO2 emissions should be considered distinct from the biogenic CO2 that arises from cellular respiration, though this distinction is not made in current GHG accounting practices. The aim of this study was to evaluate the performance of two experimental systems operated under autotrophic mode and buffered with bicarbonate, to investigate the relationship between ammonia removal and gaseous CO2 emissions. The first system consisted of continuously aerated lab-scale batch reactors, which were effective in demonstrating the important link between ammonia oxidizer activity, pH, and gaseous CO2 production. Depletion of the buffer system always led to a rapid decline in system pH and cessation of CO2 emissions when the pH fell below 7.0. The second system was a tubular continuous-flow biofilm reactor which permitted comparison of ammonia removal and CO2 emission rates. A linear relationship between ammonia removal and CO2 emissions was demonstrated and the quantified CO2 production was relatively close to that which was predicted based on the stoichiometry of nitrification, with this CO2 being detected in the gas phase. It was apparent that this system offered minimal resistance to the mass transfer of CO2 from the liquid to gas, which is an important factor that determines how much of the bicarbonate-derived CO2 may contribute to greenhouse gas emissions in engineered systems such as those used for BNR. Highlights: CO2 emission was measured during autotrophic ammonia removal under batch and continuous flow. Linear relationship found between rate of ammonia removal and CO2 emission. Depletion of bicarbonate alkalinity was identified as main source of emitted CO2 . This phenomenon should be considered during GHG accounting of BNR systems.