Short chain fatty acids released by Fusobacterium nucleatum are neutrophil chemoattractants acting via free fatty acid receptor 2 (FFAR2). (11th May 2021)

• Record Type: Journal Article
• Authors: Dahlstrand Rudin, Agnes; Khamzeh, Arsham; Venkatakrishnan, Vignesh; Basic, Amina; Christenson, Karin; Bylund, Johan
• Is Part Of: Cellular microbiology. Volume 23:Number 8(2021)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2021-05-11
• Subjects: acetate -- FFAR2 -- GPR43 -- granulocyte -- periodontitis -- PMN -- propionate -- SCFA; Microbiology -- Periodicals; Cytology -- Periodicals; Host-parasite relationships -- Periodicals; Microbiology -- Periodicals; Cells -- Periodicals; Microbiologie -- Périodiques; Microbiologie; Relation hôte-parasite; Cytologie; Cellule; Réponse cellulaire; Ressource Internet (Descripteur de forme); Périodique électronique (Descripteur de forme); 579.05
• DOI: 10.1111/cmi.13348
• Languages: English
• ISSNs: 1462-5814

Abstract

Abstract: Fusobacterium nucleatum is a gram‐negative and anaerobic oral commensal that is implicated in inflammatory conditions of the tooth‐supporting structures, that is, periodontal diseases. One of the main characteristics of these conditions is an accumulation of neutrophil granulocytes in the gingival pockets where bacteria reside. Neutrophils are recruited to tissue‐residing microbes by gradients of bacteria derived chemoattractants, and the cellular migration over the pocket epithelium into the gingival pocket is likely governed by chemoattractants released by the amino acid fermenting anaerobes typically colonising this site. However, the chemoattractants released by F. nucleatum and other oral anaerobes have long been unidentified. In the present study, we show that the major chemoattractants released during the growth of F. nucleatum are short chain fatty acids (SCFAs), primarily acetate and butyrate. These SCFAs, that are released at high levels as end‐products of the metabolism of F. nucleatum, trigger chemotaxis of human neutrophils, as well as cytosolic Ca 2+ signals, via free fatty acid receptor 2 (FFAR2). This finding establishes the SCFA‐FFAR2 interaction as an important mechanism in the recruitment of neutrophils to the periodontal pocket, but could also be of importance in the pathogenesis of other medical conditions involving colonisation/infection of F. nucleatum . Abstract : The present study shows that short chain fatty acids (SCFAs) derived from amino acid fermentation of the oral commensal F. nucleatum trigger neutrophil chemotaxis and cytosolic Ca 2+ signals via free fatty acid receptor 2 (FFAR2), and that these SCFAs are the main chemoattractants released during growth of this bacterium.