Profiling pharmacokinetics of double‐negative T cells and cytokines via a single intravenous administration in NSG mice. (3rd July 2021)

• Record Type: Journal Article
• Authors: Xu, Shangzhi; Ge, Xinyu; Wang, Liuyang; Tao, Yimin; Tang, Dongmei; Deng, Xiaojie; Yang, Fei; Zhang, Qian; Qi, Xinming; Gong, Likun; Yang, Liming
• Is Part Of: Biopharmaceutics & drug disposition. Volume 42:Number 7(2021)
• Page Start: 338
• Page End: 347
• Publication Date: 2021-07-03
• Subjects: cytokine -- double negative T cell -- pharmacodynamics -- pharmacokinetics; Biopharmaceutics -- Periodicals; Drugs -- Metabolism -- Periodicals; Pharmacology -- Periodicals; Biopharmaceutics -- Periodicals; Pharmaceutical Preparations -- metabolism -- Periodicals; 615.19
• DOI: 10.1002/bdd.2295
• Languages: English
• ISSNs: 0142-2782

Abstract

Abstract: This study was intended to delineate the profile of double‐negative T cells (DNTs) in NOD.Cg‐Prkdc scid Il2rg tm1wj /SzJ mice and cytokines released from DNTs in vivo and in vitro. Total 4 × 10 7 cells of RC1012 injection per mice were intravenously infused. IFN‐γ, TNF‐α, IL‐1β, IL‐2, IL‐4, IL‐6, IL‐10 were measured in vivo and in vitro. A quantitative polymerase chain reaction (PCR) was employed to determine the gene copies of Notch2‐NLA per DNT cell from collected organs. Cytokines were significantly increased in vitro (4 h) and in vivo (3 h). DNT cells were distributed into the lung, liver, heart, and kidney earlier, and redistributed to lymphocyte homing spleen and bone marrow, which seemed to frame a two‐compartment pharmacokinetics (PK) model but more data are needed to confirm this, and the clearance of DNT cells fell into first‐order kinetics. Abstract :